Italy make numerous changes after their disappointing opening draw, while Spain rely on Gonzalo Villar and drop Brahim Diaz in the European Under-21 Championship.
It kicks off at 20.00 GMT.
Spain opened their group with a 3-0 win over Slovenia, while the Azzurrini drew 1-1 with the Czech Republic when down to nine men.
Because of the cards in that encounter, Sandro Tonali was given a three-match ban for his stamp, with Riccardo Marchizza and Matteo Gabbia are suspended for one round.
Gianluca Scamacca scored in the first game and the Genoa striker, on loan from Sassuolo, leads the line with Patrick Cutrone again.
Roma midfielder Gonzalo Villar was on target for Spain and he plays again today, but Milan’s Brahim Diaz drops to the bench.
It’s reported this is not so much a tactical choice as the fact he received a knock to the foot during the last game.
In the other match for this group, Slovenia and the Czech Republic drew 1-1 this afternoon.
Spain U21: Fernandez; Mingueza, Hugo Guillamon, Jorge Cuenca, Miranda; M Garcia, Zubimendi, Villar; Puado, Ruiz, Cucurella
Spain bench: Martinez, Dominguez, Pipa, Pedrosa, Pozo, Fran Beltran, Moncayola, Brahim Diaz, Dani Gomez, Ricard Puig, Barrenetxea, Pino
Italy U21: Carnesecchi; Lovato, Del Prato, Ranieri; Bellanova, Frattesi, Rovella, Pobega, Frabotta; Scamacca, Cutrone
Italy bench: Plizzari, Cerofolini, Colombo, Pirola, Zappa, Sala, Raspadori, Maggiore
Ref: Osmers (GER)